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millipore轉印膜

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產品名稱: millipore轉印膜
產品型號: IPVH08130
產品展商: millipore
產品價格: 0.00 元
產品文檔: 無相關文檔

簡單介紹

millipore轉印膜IPVH08130 Millipore 提供四種不同的不動 PVDF 膜，每種膜都針對不同的蛋白質印跡應用進行優化。 millipore轉印膜IPVH08130 孔徑0.45 微米


millipore轉印膜  的詳細介紹

 millipore轉印膜IPVH08130 

Millipore 提供三種不同的Immobilon PVDF膜，是針對不同的蛋白質印跡應用的理想選擇。現在我們還提供采用按規格裁切的印跡方片膜和兩層過濾紙組成的印跡三明治。

與硝酸纖維素膜相比，Immobilon膜具有多種優點，它們不易破裂或卷曲，便于裁剪且在切割過程中不會碎裂。它們還有低背景，廣泛溶劑相容性以及優異的著色能力。此外，它們可以重復檢測。

Description
Catalogue Number IPVH08130
Trade Name
  • Immobilon
Description Immobilon-P PVDF Membrane
Background Information Overview
- Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

Immobilon-P Transfer Membrane:
- 45 μm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
- Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 μm pore size recommended for most western blotting, especially proteins greater than 20 kDa

Blotting Sandwiches:
- When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

Blotting Filter Paper:
- Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

Immobilon-PSQ Membrane:
- 0.2 μm pore size and a large internal structure
- Higher protein adsorption and sequencing yields than other membranes
- Recommended for blotting proteins less than 20 kDa
- Prevents blow-through of low molecular weight proteins

Immobilon-FL Membrane:
- The first transfer membrane optimized for fluorescence applications
- Extremely low background improves sensitivity of all fluorescence detection protocols
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
- Ideal for multiplexing and chemifluorescence applications

Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.

Features & Benefits:
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be reprobed multiple times

Applications:
- Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
- Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
References

Product Information
Detection method Chromogenic Radioactive Fluorescent Chemiluminescence
Compatible Stains
  • Coomassie brilliant blue
  • Amido black
  • India ink
  • Ponceau-S red
  • Colloidal gold
  • CPTS
  • Toluidine blue
  • Transillumination
  • Sypro® ruby
Filter Code IPVH
Filter Color White

Applications
Application 10 sheets, 8.5 cm x 13.5 cm, 0.45 μm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
Key Applications
  • Western Blotting

Biological Information
Primary Binding Mechanisms Electrostatic, hydrophobic
BSA Adsorption 215 μg/cm2
Goat IgG Adsorption 294 μg/cm2
Insulin Adsorption 160 μg/cm2
Wettability Hydrophobic

Physicochemical Information
Pore Size 0.45 μm

Dimensions
Filter Surface Plain
Filter Dimensions 8.5 cm x 13.5 cm

Materials Information
Chemistry
  • Hydrophobic Polyvinylidene Fluoride (PVDF)
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information

Packaging Information
Material Size 10

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